7Q8A

Crystal structure of tandem domain RRM1-2 of FUBP-interacting repressor (FIR) bound to FUSE ssDNA fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999998
Spacegroup name	P 21 21 21
Unit cell lengths	55.696, 69.785, 107.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.500 - 2.050
R-factor	0.2008
Rwork	0.199
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qfj
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20-4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.500	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.074	0.520
Number of reflections	26557	1979
<I/σ(I)>	9
Completeness [%]	98.9
Redundancy	4.3
CC(1/2)	0.997	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	25% w/v PEG3350, 0.2M Sodium acetate trihydrate, 0.1M Bis-Tris , pH5.5