7Q89
OleP mutant G92W in complex with 6DEB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 247.758, 110.489, 159.818 |
| Unit cell angles | 90.00, 129.66, 90.00 |
Refinement procedure
| Resolution | 48.030 - 2.080 |
| R-factor | 0.17331 |
| Rwork | 0.170 |
| R-free | 0.22779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mns |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.857 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.080 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.080 | |
| Number of reflections | 462764 | 227594 |
| <I/σ(I)> | 7.34 | |
| Completeness [%] | 98.8 | 97.8 |
| Redundancy | 3.4 | 3 |
| CC(1/2) | 0.997 | 0.323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Sodium Formate 4.4M |
