7Q83

Crystal structure of S. cerevisiae Sso2 in complex with the pleckstrin homology domain of Sec3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	50.961, 58.402, 83.286
Unit cell angles	104.28, 98.49, 113.20

Refinement procedure

Resolution	19.950 - 2.190
R-factor	0.1988
Rwork	0.197
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m4y
RMSD bond length	0.002
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.270
High resolution limit [Å]	2.190	2.190
Rmerge	0.164	1.094
Rmeas	0.189
Rpim	0.093	0.636
Number of reflections	40853	3771
<I/σ(I)>	6.73	1.18
Completeness [%]	96.1	89.79
Redundancy	4	3.9
CC(1/2)	0.990	0.438

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.1 M sodium acetate (pH 5.0), 0.2 M ammonium acetate, and 30% (w/v) PEG 4,000