7Q7Z
Crystal structure of the methyltransferase-ribozyme 1 (with 1-benzylamine-adenosine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 117.861, 117.861, 83.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.540 - 3.260 |
R-factor | 0.2132 |
Rwork | 0.210 |
R-free | 0.26450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7q7x |
RMSD bond length | 0.001 |
RMSD bond angle | 0.303 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.540 | 3.380 |
High resolution limit [Å] | 3.260 | 3.260 |
Rmerge | 0.140 | |
Number of reflections | 9584 | 937 |
<I/σ(I)> | 11.9 | 0.6 |
Completeness [%] | 99.7 | 98.83 |
Redundancy | 15.4 | |
CC(1/2) | 0.995 | 0.236 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 36-42% MPD, 50mM MES pH 6.4-6.7, 100mM NaCl, 100mM LiCl, 10mM MgCl2 |