7Q7Z

Crystal structure of the methyltransferase-ribozyme 1 (with 1-benzylamine-adenosine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 41 2 2
Unit cell lengths	117.861, 117.861, 83.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.540 - 3.260
R-factor	0.2132
Rwork	0.210
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7q7x
RMSD bond length	0.001
RMSD bond angle	0.303
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.540	3.380
High resolution limit [Å]	3.260	3.260
Rmerge	0.140
Number of reflections	9584	937
<I/σ(I)>	11.9	0.6
Completeness [%]	99.7	98.83
Redundancy	15.4
CC(1/2)	0.995	0.236

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	36-42% MPD, 50mM MES pH 6.4-6.7, 100mM NaCl, 100mM LiCl, 10mM MgCl2