7Q7P

LIPIDIC CUBIC PHASE SERIAL FEMTOSECOND CRYSTALLOGRAPHY STRUCTURE OF A PHOTOSYNTHETIC REACTION CENTRE

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	293
Detector technology	CCD
Collection date	2018-11-01
Detector	MPCCD
Wavelength(s)	1.4
Spacegroup name	P 21 21 2
Unit cell lengths	84.900, 125.300, 182.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.920 - 2.400
Rwork	0.165
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i5n
RMSD bond length	0.014
RMSD bond angle	2.152
Data reduction software	CrystFEL (0.6.3)
Data scaling software	CrystFEL (0.6.3)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.920	2.420
High resolution limit [Å]	2.400	2.400
Number of reflections	76977	76977
<I/σ(I)>	7.87
Completeness [%]	100.0
Redundancy	7.05
CC(1/2)	0.994	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	60:40 monoolein with UQ2, protein 40 mg/mL concentration.