7Q72

Structure of Pla1 in complex with Red1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 2
Unit cell lengths	128.644, 151.337, 65.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.017 - 2.800
R-factor	0.2448
Rwork	0.241
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	deposition ID D_1292115151
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (1.19.2_4158+SVN)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.017	98.017	2.970
High resolution limit [Å]	2.800	12.930	2.890
Rmerge	0.065	0.059	4.371
Rmeas	0.103	0.063	4.546
Number of reflections	20986	383	2023
<I/σ(I)>	9.91	29.2	0.58
Completeness [%]	97.3	98.5	95.1
Redundancy	13.164	9.77	13.365
CC(1/2)	0.998	0.992	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M potassium formate, 20% PEG 3350