7Q71
The crystallographic structure of the Ligand Binding domain of the NR7 nuclear receptor from the amphioxus Branchiostoma lanceolatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 46.290, 46.290, 163.017 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.930 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xiu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.750 | 40.750 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.950 | 2.000 |
| Rmerge | 0.037 | 0.020 | 0.966 |
| Rmeas | 0.040 | 0.022 | 1.046 |
| Rpim | 0.015 | 0.009 | 0.395 |
| Total number of observations | 85112 | 1022 | 6585 |
| Number of reflections | 13203 | 188 | 982 |
| <I/σ(I)> | 22 | 68.9 | 1.9 |
| Completeness [%] | 94.5 | 99.8 | 100 |
| Redundancy | 6.4 | 5.4 | 6.7 |
| CC(1/2) | 1.000 | 1.000 | 0.682 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.3 M Li2SO4, 0.1 M HEPES pH 7.5 |
