7Q6D

E. coli FtsA 1-405 ATP 3 Ni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97948
Spacegroup name	P 1 21 1
Unit cell lengths	51.102, 64.177, 65.285
Unit cell angles	90.00, 96.07, 90.00

Refinement procedure

Resolution	45.640 - 2.800
R-factor	0.2194
Rwork	0.217
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3WQT (without IC domain)
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.640	45.640	2.850
High resolution limit [Å]	2.700	8.540	2.700
Rmerge		0.040	0.863
Rmeas	0.169	0.047	1.027
Rpim	0.091	0.026	0.552
Total number of observations	39110	1211	5673
Number of reflections	11651	388	1683
<I/σ(I)>	6.8	21.2	1.4
Completeness [%]	99.8	98.4	99.9
Redundancy	3.4	3.1	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	20 % w/v PEG 4K, 0.175 M (NH4)2SO4, 5 mM Ni(II)Cl2, 0.1 M Tris/HAc pH 7.5, 0.017 M MES/NaOH pH 5.9