7Q68
Crystal structure of Chaetomium thermophilum wild-type Ahp1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 75.249, 75.249, 110.241 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.920 - 1.750 |
R-factor | 0.1885 |
Rwork | 0.188 |
R-free | 0.20190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dsr |
RMSD bond length | 0.005 |
RMSD bond angle | 0.584 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.920 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.067 | 1.883 |
Rmeas | 0.071 | 1.991 |
Number of reflections | 32659 | 5130 |
<I/σ(I)> | 14.81 | 0.84 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 9.329 | 9.456 |
CC(1/2) | 0.998 | 0.719 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 2 M Ammonium Sulfate and 0.1 M Sodium cacodylate -pH 6.5. |