Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.343, 71.343, 200.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.304 - 2.717 |
| Rwork | 0.274 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iwv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.858 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.304 | 2.788 |
| High resolution limit [Å] | 2.717 | 2.717 |
| Rmeas | 0.025 | 0.830 |
| Number of reflections | 14670 | 980 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 294 | 30-60 mM Sodium Citrate, 50-70mM NaCl, 12-14 % (w/v) PEG-3350, 60-65 mM n-octyl-beta-d-glucoside, 6-12 mg/mL protein |
