7Q50

human Gid4 bound to a Phe/N-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-09-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 41 3 2
Unit cell lengths	131.307, 131.307, 131.307
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.420 - 3.160
R-factor	0.2393
Rwork	0.236
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7q4y
RMSD bond length	0.005
RMSD bond angle	0.760
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.420	3.350
High resolution limit [Å]	3.160	3.160
Number of reflections	12581	1981
<I/σ(I)>	9.1
Completeness [%]	99.6
Redundancy	20.6
CC(1/2)	0.994	0.206

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.1 M Sodium malonate, 0.3% Jeffamine ED-2001 pH 7, 0.1 M HEPES pH 7