7Q4Y
human Gid4 bound to a Gly/N-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-04-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 72.023, 72.023, 146.316 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.370 - 3.080 |
| R-factor | 0.251 |
| Rwork | 0.244 |
| R-free | 0.26450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cdc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.486 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.160 | 3.260 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Number of reflections | 15664 | 2435 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.994 | 0.236 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 18% PEG 3350, 0.2 M ammonium nitrate, 0.1 M Bis-Tris pH 7 |
