7Q48
Solution structure of an intramolecular RNA G-quadruplex formed by the 6A8U17U mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto onco-gene
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 600 | |
| 2 | Bruker | AVANCE | 700 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-1H NOESY |
| 10 | 4 | 4 | long-range 1H-15N HSQC |
| 11 | 5 | 5 | long-range 1H-15N HSQC |
| 12 | 6 | 6 | constant-time 1H-13C HSQC |
| 13 | 7 | 7 | constant-time 1H-13C HSQC |
| 14 | 8 | 8 | constant-time 1H-13C HSQC |
| 15 | 9 | 9 | constant-time 1H-13C HSQC |
| 2 | 2 | 2 | 2D 1H-1H NOESY |
| 3 | 2 | 2 | 2D 1H-1H TOCSY |
| 4 | 2 | 2 | 2D 1H-13C HSQC |
| 5 | 3 | 3 | 2D 1H-15N HSQC |
| 6 | 1 | 1 | JR-HMBC |
| 7 | 3 | 3 | H8N2 HNN-COSY |
| 8 | 3 | 3 | H1N2 HNN-COSY |
| 9 | 2 | 2 | H1pC6/8 HMBC |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 7 | 1 | 298 |
| 2 | 7 | 1 | 298 |
| 3 | 7 | 1 | 298 |
| 4 | 7 | 1 | 298 |
| 5 | 7 | 1 | 298 |
| 6 | 7 | 1 | 298 |
| 7 | 7 | 1 | 298 |
| 8 | 7 | 1 | 298 |
| 9 | 7 | 1 | 298 |
Conformers
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 20 |
