7Q47

Endolysin from bacteriophage Enc34, catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	65.304, 128.993, 36.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.890 - 1.600
R-factor	0.179
Rwork	0.177
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Enc34 ORF39-SeMet
RMSD bond length	0.009
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.890	45.890	1.700
High resolution limit [Å]	1.600	5.000	1.600
Rmerge	0.168	0.089	1.525
Rmeas	0.175	0.093	1.597
Total number of observations	503383
Number of reflections	41411	1507	6765
<I/σ(I)>	9.9	21.94	2.03
Completeness [%]	100.0	99.7	100
Redundancy	12.156	11.565	11.394
CC(1/2)	0.996	0.995	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1 M phosphate/citrate, 0.2 M sodium chloride, 20% w/v PEG 8000