7Q43
Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of dedicator of cytokinesis protein 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-23 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.999997 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 108.820, 108.820, 243.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.665 - 2.400 |
R-factor | 0.205598267093 |
Rwork | 0.202 |
R-free | 0.26497 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kci |
RMSD bond length | 0.008 |
RMSD bond angle | 0.911 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASES |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.665 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 57945 | 4230 |
<I/σ(I)> | 5.77 | |
Completeness [%] | 99.7 | |
Redundancy | 37.6713780309 | |
CC(1/2) | 0.983 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | hanging drop 20% PEG 3350 0.18M tris amonium citrate |