7Q42

Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of chromatin reader BAZ2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978564
Spacegroup name	P 43 21 2
Unit cell lengths	107.980, 107.980, 241.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 1.950
R-factor	0.16974605582
Rwork	0.168
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.011
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	104649	7616
<I/σ(I)>	10.93	1.03
Completeness [%]	99.9
Redundancy	27.0621601735
CC(1/2)	0.998	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	hanging drop 20% PEG 3350 0.18M tris amonium citrate