7Q3A
Crystal structure of MAB_4324 a tandem repeat GNAT from Mycobacterium abscessus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-31 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 149.960, 149.960, 183.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.090 - 2.000 |
R-factor | 0.1871 |
Rwork | 0.186 |
R-free | 0.22570 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.994 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRANK |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.100 | 0.870 |
Number of reflections | 80802 | 8058 |
<I/σ(I)> | 37.4 | |
Completeness [%] | 98.3 | 100 |
Redundancy | 38.3 | |
CC(1/2) | 1.000 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 2 M Ammonium sulphate, 0.1 M HEPES pH 7.5 and 2% w/v polyethylene glycol (PEG) 400. |