7Q39

Ribonucleotide Reductase R2_genomic protein from Aquifex aeolicus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	69.246, 69.246, 177.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.247 - 2.100
Rwork	0.238
R-free	0.29730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ail
RMSD bond length	0.006
RMSD bond angle	1.352
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.247	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	26173	2084
<I/σ(I)>	22.9
Completeness [%]	100.0
Redundancy	25.7
CC(1/2)	1.000	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M citrate pH 4.0, 1.0 M lithium chloride, 20 % PEG6000