7Q38
Crystal structure of the mutant bacteriorhodopsin pressurized with argon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97241 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 115.657, 119.245, 36.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.020 - 1.650 |
| R-factor | 0.16452 |
| Rwork | 0.162 |
| R-free | 0.20522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xxj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.810 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.050 | 1.769 |
| Number of reflections | 57738 | 4476 |
| <I/σ(I)> | 16.29 | 0.92 |
| Completeness [%] | 99.0 | 99.4 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.999 | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 3.0 M Ammonium Sulfate,0.1 M Sodium Acetate, pH 4.6 |
