7Q36
Crystal structure of KR2 sodium pump rhodopsin pressurized with krypton
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8637 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.489, 82.180, 233.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 116.720 - 2.600 |
| R-factor | 0.21902 |
| Rwork | 0.216 |
| R-free | 0.26904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xtl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.720 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.177 | 2.000 |
| Rmeas | 0.192 | 2.117 |
| Number of reflections | 23274 | 4905 |
| <I/σ(I)> | 9.29 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.998 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 2.0 M sodium malonate, pH 4.3 |
