7Q2T

Crystal structure of untagged rat C2orf32 (also known as CNRIP1) in a domain-swapped conformation

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.83000
Spacegroup name	C 2 2 21
Unit cell lengths	29.250, 79.600, 130.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.800 - 1.651
Rwork	0.168
R-free	0.21800
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.661
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.800	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.077	0.811
Rmeas	0.081	0.878
Rpim	0.024	0.325
Number of reflections	18699	829
<I/σ(I)>	23	2.2
Completeness [%]	99.2	90.7
Redundancy	11.2	6.86
CC(1/2)	0.999	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5-10% PEG1500, SPG buffer pH 6.0-6.5, 5-10mM DTT