7Q1Y
X-ray structure of human A2ML1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 320.150, 320.150, 321.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.370 - 4.400 |
| R-factor | 0.2397 |
| Rwork | 0.238 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | densities from EM map |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.881 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 211.000 | 4.380 |
| High resolution limit [Å] | 4.276 | 4.276 |
| Rmerge | 0.301 | |
| Number of reflections | 43680 | 2976 |
| <I/σ(I)> | 8.75 | |
| Completeness [%] | 99.4 | 93.3 |
| Redundancy | 58.7 | |
| CC(1/2) | 0.998 | 0.261 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Potassium phosphate dibasic, 10 % PEG 3350 pH 9.2 |
