7Q1L
Glycosilated Human Serum Apo-tranferrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.631, 102.148, 199.969 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.350 - 3.000 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hav |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.853 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19-4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.350 | 37.350 | 3.130 |
| High resolution limit [Å] | 3.000 | 10.390 | 3.000 |
| Rmerge | 0.096 | 0.040 | 1.606 |
| Rmeas | 0.104 | 0.043 | 1.718 |
| Rpim | 0.038 | 0.016 | 0.606 |
| Total number of observations | 267933 | 6467 | 35356 |
| Number of reflections | 36713 | 979 | 4437 |
| <I/σ(I)> | 9.9 | 31.2 | 1.3 |
| Completeness [%] | 99.9 | 98 | 100 |
| Redundancy | 7.3 | 6.6 | 8 |
| CC(1/2) | 0.999 | 0.999 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.5 | 0.2 M Ammonium Citrate pH 7.0, 20% PEG 3350 |
