7Q1G

Crystal structure and metal binding properties of the periplasmic iron component EfeM from Pseudomonas syringae EfeUOB/M iron-transport system

Replaces:  6SNGReplaces:  4BGO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	P 2 21 21
Unit cell lengths	46.800, 95.230, 152.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.804 - 1.600
R-factor	0.1863
Rwork	0.185
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3at7
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.800	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.071	0.625
Rmeas	0.083	0.737
Rpim	0.043	0.384
Number of reflections	90374	13064
<I/σ(I)>	10.6	2
Completeness [%]	99.4	99.6
Redundancy	3.5	3.6
CC(1/2)	0.997	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium sulphate, lithium sulphate, Tris