7PZ0
Structure of LPMO (expressed in E.coli) with cellotriose at 9.81x10^6 Gy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-03 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 41 |
Unit cell lengths | 48.900, 48.900, 108.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.650 - 1.200 |
R-factor | 0.1347 |
Rwork | 0.134 |
R-free | 0.14750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7pqr |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 142855 | 10325 |
<I/σ(I)> | 19.53 | |
Completeness [%] | 90.6 | |
Redundancy | 1.61 | |
CC(1/2) | 0.990 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 4.5 | 278 | 2.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 under silicon:paraffin oil in a 3:2 ratio |