7PYY

Structure of LPMO (expressed in E.coli) with cellotriose at 5.05x10^5 Gy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0332
Spacegroup name	P 41
Unit cell lengths	48.850, 48.850, 108.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.600 - 1.200
R-factor	0.1451
Rwork	0.144
R-free	0.15780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pqr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.230
High resolution limit [Å]	1.200	1.200
Number of reflections	141907	10137
<I/σ(I)>	8.78
Completeness [%]	90.2
Redundancy	1.72
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.5	278	2.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 under silicon:paraffin oil in a 3:2 ratio