7PYO
Structure of an LPMO (expressed in E.coli) at 2.31x10^5 Gy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-03 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.860, 48.860, 108.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.620 - 1.400 |
| R-factor | 0.1393 |
| Rwork | 0.138 |
| R-free | 0.16320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7pqr |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 92581 | 6814 |
| <I/σ(I)> | 9.43 | |
| Completeness [%] | 93.4 | |
| Redundancy | 1.6 | |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.5 | 278 | 2.3 M Ammonium sulfate, 0.1 M Sodium acetate pH 4.5 under Silicon:Paraffin oil in a 3:2 ratio. |
