7PXW
LPMO, expressed in E.coli, in complex with Cellotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.770, 48.770, 109.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.580 - 1.400 |
| R-factor | 0.1193 |
| Rwork | 0.117 |
| R-free | 0.15980 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ach |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.837 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.580 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmeas | 0.117 | 3.417 |
| Number of reflections | 49868 | 3724 |
| <I/σ(I)> | 12.07 | |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 13.62 | 13.57 |
| CC(1/2) | 0.999 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.2 | 277 | 2.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.2. Crystals were soaked in 0.05 M cellotetraose in crystallization conditions with 18% glycerol for 30 min. |
