7PXR

Room temperature structure of an LPMO.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-08-13
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.540
Spacegroup name	P 41 3 2
Unit cell lengths	126.659, 126.659, 126.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.870 - 1.800
R-factor	0.1478
Rwork	0.147
R-free	0.16620
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5ach
RMSD bond length	0.014
RMSD bond angle	1.844
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.870	1.840
High resolution limit [Å]	1.800	1.800
Rmeas	0.047	0.509
Number of reflections	32798	1930
<I/σ(I)>	43.8	4.8
Completeness [%]	100.0	100
Redundancy	17.2	10.2
CC(1/2)	1.000	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	298	3.0 M sodium chloride, 0.1 M citric acid pH 3.5 The crystals were soaked in a drop containing 3.0 M sodium chloride, 0.1 M citric acid pH 5.5 for 10 min before mounting