7PXQ

GH115 alpha-1,2-glucuronidase D303A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 61 2 2
Unit cell lengths	148.150, 148.150, 274.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.470 - 2.300
R-factor	0.1918
Rwork	0.190
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c90
RMSD bond length	0.008
RMSD bond angle	0.901
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.470	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	79371	7781
<I/σ(I)>	17.3
Completeness [%]	99.9
Redundancy	20
CC(1/2)	1.000	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M CaCl2, 0.1 M HEPES pH 7.5 and 28% PEG400