7PWE
Crystal structure of the glutaredoxin/ferredoxin disulfide reductase fusion protein from Desulfotalea psychrophila Lsv54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979110 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.600, 49.300, 86.000 |
| Unit cell angles | 90.00, 116.50, 90.00 |
Refinement procedure
| Resolution | 41.420 - 2.077 |
| R-factor | 0.1926 |
| Rwork | 0.191 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.420 | 2.130 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmeas | 0.037 | 0.236 |
| Number of reflections | 54939 | 4067 |
| <I/σ(I)> | 22.81 | 5.84 |
| Completeness [%] | 98.7 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 277 | 30% MPD, 10% PEG4000, and 0.1M Imidazole-HCl (JBScreen Classic condition JBS6 B2) |
