7PV7
Crystal structure of dimeric Porphyromonas gingivalis PorX, a type 9 secretion system response regulator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.830, 77.080, 138.730 |
| Unit cell angles | 82.26, 81.36, 76.41 |
Refinement procedure
| Resolution | 68.400 - 2.410 |
| Rwork | 0.186 |
| R-free | 0.21550 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.400 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.126 | 0.883 |
| Rmeas | 0.149 | 1.039 |
| Rpim | 0.078 | 0.541 |
| Number of reflections | 92407 | 8754 |
| <I/σ(I)> | 6.45 | |
| Completeness [%] | 97.9 | 93.11 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.992 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM glycine pH 8.0, 100 mM sodium formate and a PEG-mixture of 20K, 10K, 8K and 6K (1.5% each) |
