7PV6

CARM1 in complex with EML734

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.967
Spacegroup name	P 21 21 2
Unit cell lengths	75.000, 99.519, 208.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.170 - 2.400
R-factor	0.1934
Rwork	0.191
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.400	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.129	2.017
Rmeas	0.140	2.177
Rpim	0.053	0.813
Number of reflections	61974	4383
<I/σ(I)>	11.3	1.1
Completeness [%]	99.7	96.8
Redundancy	6.8	7
CC(1/2)	0.999	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20 mM Tris pH 7.5, 50 mM NaCl, 1 mM TCEP