7PV5
RBD domain of D. melanogaster tRNA (uracil-5-)-methyltransferase homolog A (TRMT2A)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-30 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0366445605250807 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.410, 41.890, 48.820 |
| Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
| Resolution | 45.640 - 1.600 |
| R-factor | 0.1436 |
| Rwork | 0.141 |
| R-free | 0.19930 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.936 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 23209 | 1684 |
| <I/σ(I)> | 11.63 | 3.51 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.998 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 2M AmSO4 and 2M NaCl |
