7PV0

Crystal structure of a Mic60-Mic19 fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	43.815, 48.517, 70.739
Unit cell angles	82.70, 79.14, 72.41

Refinement procedure

Resolution	46.120 - 2.150
R-factor	0.2512
Rwork	0.250
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alpha-helices
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMPLE
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.120	46.120	2.280
High resolution limit [Å]	2.150	6.390	2.150
Rmerge	0.071	0.023	0.881
Rmeas	0.090	0.029	1.115
Total number of observations	68282
Number of reflections	27300	1026	4445
<I/σ(I)>	8.28	33.09	0.92
Completeness [%]	91.8	91	92.3
Redundancy	2.501	2.501	2.496
CC(1/2)	0.998	0.999	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	33% (v/v) Jeffamine M-600, 0.1 M HEPES/NaOH pH 7.2