7PUH
Crystal Structure of Two-Domain Laccase mutant H165A/R240H from Streptomyces griseoflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.760, 94.286, 120.482 |
| Unit cell angles | 90.00, 91.27, 90.00 |
Refinement procedure
| Resolution | 47.410 - 1.300 |
| R-factor | 0.1338 |
| Rwork | 0.133 |
| R-free | 0.15690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lhl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.380 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 405781 | 64031 |
| <I/σ(I)> | 18.19 | 1.28 |
| Completeness [%] | 99.1 | 97 |
| Redundancy | 6.76 | 6.35 |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate condition #31 of BCS-1 from Molecular Dimensions, UK |
