7PU9
Crystal structure of CaM in complex with CDZ (form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.8266 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 39.351, 39.351, 336.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.150 - 2.279 |
R-factor | 0.2358 |
Rwork | 0.234 |
R-free | 0.26630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ctr |
RMSD bond length | 0.008 |
RMSD bond angle | 0.880 |
Data reduction software | AutoProcess |
Data scaling software | AutoProcess |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.153 | 2.318 |
High resolution limit [Å] | 2.279 | 2.279 |
Rmerge | 0.156 | 2.112 |
Rmeas | 0.159 | 2.139 |
Rpim | 0.027 | 0.334 |
Number of reflections | 8009 | 395 |
<I/σ(I)> | 14.9 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 34.8 | |
CC(1/2) | 1.000 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292 | 0.2 M CaCl2, 0.1 M Tris pH 8.5 and 25 %w/v PEG 4K |