7PU4
Crystal structure of the dimer RBP-N and RBP-Trunc from Thermotoga maritima Ribose Binding Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-26 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.210, 84.200, 103.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.010 - 1.690 |
R-factor | 0.2073 |
Rwork | 0.206 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fn9 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.892 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.010 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.122 | 1.800 |
Rmeas | 0.127 | 1.196 |
Rpim | 0.035 | 1.104 |
Number of reflections | 64629 | 6298 |
<I/σ(I)> | 14.25 | 0.96 |
Completeness [%] | 99.8 | 98.39 |
Redundancy | 13.3 | 12.8 |
CC(1/2) | 0.999 | 0.318 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M HEPES pH: 7.5, PEG 20000 15% w/v, RBP-N and RBP-Trunc equimolarly mixed at 0.5 mM |