7PU0

Crystal Structure of Two-Domain Laccase mutant H165A/M199G from Streptomyces griseoflavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	120
Detector technology	PIXEL
Collection date	2020-12-18
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	76.235, 94.591, 120.867
Unit cell angles	90.00, 91.39, 90.00

Refinement procedure

Resolution	24.740 - 2.200
R-factor	0.1585
Rwork	0.157
R-free	0.18850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lhl
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.190	1.070
Number of reflections	85543	4591
<I/σ(I)>	6.1	1.5
Completeness [%]	98.1	98.7
Redundancy	3.7
CC(1/2)	0.980	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate