7PTZ

High resolution X-ray structure of E. coli expressed Lentinus similis LPMO.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 41
Unit cell lengths	48.659, 48.659, 109.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 1.093
Rwork	0.123
R-free	0.13930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pqr
RMSD bond length	0.020
RMSD bond angle	2.032
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	1.160
High resolution limit [Å]	1.090	1.090
Rmeas	0.059
Number of reflections	96541	10733
<I/σ(I)>	19.3
Completeness [%]	91.3
Redundancy	11.99
CC(1/2)	0.990	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	0.1 M citric acid pH 3.5 and 3.0 M NaCl