7PTZ
High resolution X-ray structure of E. coli expressed Lentinus similis LPMO.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 |
Unit cell lengths | 48.659, 48.659, 109.593 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.500 - 1.093 |
Rwork | 0.123 |
R-free | 0.13930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7pqr |
RMSD bond length | 0.020 |
RMSD bond angle | 2.032 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 1.160 |
High resolution limit [Å] | 1.090 | 1.090 |
Rmeas | 0.059 | |
Number of reflections | 96541 | 10733 |
<I/σ(I)> | 19.3 | |
Completeness [%] | 91.3 | |
Redundancy | 11.99 | |
CC(1/2) | 0.990 | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.1 M citric acid pH 3.5 and 3.0 M NaCl |