7PTM
Crystal Structure of Two-Domain Laccase mutant M199G/R240H from Streptomyces griseoflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2021-09-16 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.064, 93.726, 119.233 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 24.680 - 1.850 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lhl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | CrysalisPro (40.84a) |
| Data scaling software | CrysalisPro (40.84a) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.164 | 1.240 |
| Rmeas | 0.192 | |
| Number of reflections | 138143 | 6533 |
| <I/σ(I)> | 6.96 | 1 |
| Completeness [%] | 99.2 | 95.9 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.980 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate |
