7PTB
Crystal structure of the SPD-2 domain of human CEP192
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-05-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97835 |
| Spacegroup name | P 61 |
| Unit cell lengths | 104.588, 104.588, 90.214 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.580 - 2.080 |
| R-factor | 0.21252 |
| Rwork | 0.212 |
| R-free | 0.22364 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.171 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.600 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rpim | 0.024 | 0.357 |
| Number of reflections | 33669 | 32002 |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292.15 | 100 nl protein solution and 100 nl of reservoir solution which was 0.1 M Na-Acetate pH 5.3, 3.9 M ammonium ni-trate. Crystals were mounted in 0.1 M Na-Acetate pH 4.6, 1 M ammonium nitrate, 30% glycerol. |
