7PSH
Crystal structure of beta-glucuronidase from Acidobacterium capsulatum at 1.24 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.911880 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 82.865, 44.149, 136.277 |
| Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
| Resolution | 74.690 - 1.240 |
| Rwork | 0.169 |
| R-free | 0.18780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g0m |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.959 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.690 | 1.260 |
| High resolution limit [Å] | 1.240 | 1.260 |
| Number of reflections | 134306 | 6247 |
| <I/σ(I)> | 11.6 | 2.2 |
| Completeness [%] | 97.2 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.998 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5 M AmSO4 1 M LiSO4 0.1 M Trisodium Citrate |
