7PRN
E1M, D97A, E99A MUTANT OF RH. BLASTICA PORIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 104.400, 104.400, 124.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.900 - 2.250 |
| R-factor | 0.215 * |
| Rwork | 0.215 |
| R-free | 0.22200 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1prn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.038 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.950 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.044 | 0.132 |
| Number of reflections | 23933 | 3524 * |
| <I/σ(I)> | 30.2 | 11.4 |
| Completeness [%] | 99.8 | 100 * |
| Redundancy | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 20 * | Kreusch, A., (1994) J.Mol.Biol., 243, 891. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | drop | n-octyltetraoxyethylene | 0.6 (%(w/v)) | |
| 5 | 1 | drop | 3 (mM) | ||
| 6 | 1 | drop | PEG600 | 10-18 (%) | |
| 7 | 1 | reservoir | PEG600 | 30-38 (%(w/v)) |
