7PRJ
Factor XII Fibronectin type II (FXII FnII) domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.2821 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.350, 40.880, 45.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.781 - 1.200 |
R-factor | 0.1944 |
Rwork | 0.192 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h8p |
RMSD bond length | 0.007 |
RMSD bond angle | 0.990 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.360 | 3.790 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 15507 | 15507 |
<I/σ(I)> | 4.02 | |
Completeness [%] | 97.3 | |
Redundancy | 4.5 | |
CC(1/2) | 0.993 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292.15 | 0.01 M Zinc chloride, 0.1 M Sodium acetate pH 5.0, 20 % (w/v) PEG 6000 |