7PQA
Crystal Structure of the Ring Nuclease 0811 mutant-S12G/K169G from Sulfolobus islandicus (Sis0811)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9779 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.930, 104.970, 78.306 |
Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
Resolution | 43.932 - 2.040 |
Rwork | 0.194 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7pq2 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.860 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.380 | 2.070 |
High resolution limit [Å] | 2.040 | 2.040 |
Number of reflections | 38798 | 1925 |
<I/σ(I)> | 18.5 | |
Completeness [%] | 97.6 | |
Redundancy | 7.1 | |
CC(1/2) | 0.990 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.1M Bis-Tris propane pH 7.5, 0.2M KSCN |