7PQ8
Crystal structure of Campylobacter jejuni DsbA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.489, 57.670, 93.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.889 - 1.329 |
| Rwork | 0.176 |
| R-free | 0.21920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7pq7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.754 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.860 | 1.410 |
| High resolution limit [Å] | 1.329 | 1.330 |
| Number of reflections | 51341 | 12965 |
| <I/σ(I)> | 8.94 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.78 | |
| CC(1/2) | 0.990 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.12 M Ethylene glycols, 0.1M Tris/BICINE pH 8.5, 50 % v/v Precipitant Mix 1 |
