7PQ6

Crystal Structure of the Ring Nuclease 0811 mutant-S12A from Sulfolobus islandicus (Sis0811)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	77.054, 103.735, 77.678
Unit cell angles	90.00, 102.99, 90.00

Refinement procedure

Resolution	60.820 - 2.670
Rwork	0.220
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pq2
RMSD bond length	0.010
RMSD bond angle	1.602
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.820	2.714
High resolution limit [Å]	2.670	2.670
Number of reflections	16673	3165
<I/σ(I)>	6.7
Completeness [%]	98.0
Redundancy	6.3
CC(1/2)	0.945	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.1M Bis-Tris propane pH 7.5, 0.2M KSCN