7PQ3
Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in complex with its post-catalytic reaction product
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.731, 56.731, 361.469 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.050 - 2.850 |
Rwork | 0.252 |
R-free | 0.29060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7pq2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.638 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.520 | 2.920 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 14426 | 823 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 93.5 | |
Redundancy | 7.8 | |
CC(1/2) | 0.990 | 0.370 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.1M Bis-Tris propane pH 7.5, 0.2M KSCN |