7PPB

2.4 angstrom crystal structure of bone morphogenetic protein receptor type II (BMPRII) extracellular domain in complex with BMP10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-23
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97950
Spacegroup name	C 1 2 1
Unit cell lengths	119.589, 46.343, 43.753
Unit cell angles	90.00, 110.57, 90.00

Refinement procedure

Resolution	42.820 - 2.400
R-factor	0.2076
Rwork	0.206
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ppa
RMSD bond length	0.002
RMSD bond angle	0.618
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.820	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.217	1.048
Rmeas	0.234	1.284
Rpim	0.089	0.508
Number of reflections	8820	887
<I/σ(I)>	6.7	1.5
Completeness [%]	99.0	96.3
Redundancy	6.9	6.1
CC(1/2)	0.993	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294.15	14% PEG 3350 0.14 M KCl